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. 2014 Aug 15;13(12):3294–3307. doi: 10.1074/mcp.M113.034363

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — GlcN causes increased O-GlcNAcylation primarily in the cytosolic and nuclear fractions. A, The effect of GlcN treatment on O-GlcNAc, OGT, and OGA protein levels in different subcellular fractions. CTD110.6 (anti-O-GlcNAc) Western blot of total lysate, crude mitochondrial, nuclear, and cytosolic fractions for control (−) and GlcN-treated (+) samples. Loading controls MEK1/2, COX IV and Nup 62 correspond to cytosolic, mitochondrial, and nuclear markers, respectively. B, mRNA regulation of OGT and OGA determined using Affymetrix arrays.