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. 2014 Aug 15;13(12):3294–3307. doi: 10.1074/mcp.M113.034363

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 3. — GlcN causes cell cycle arrest, apoptosis, ER stress and increased autophagy. A, Cell cycle phase distribution for KMH2 and RAMOS cells (black: sub-G1, dark gray: G0/G1, white: S-phase, and light gray: G2/M). B, Apoptosis assay for KMH2 and RAMOS cells. C, Increased vacuolization of KMH2 cells upon GlcN treatment. D, SQSTM1 was up-regulated after GlcN treatment (green depicts untreated and red depicts after the GlcN treatment) at both the (a) protein and (b) mRNA level after GlcN treatment. E, GRP78/HSPA5 was up-regulated following the GlcN treatment (green depicts untreated and red depicts after the GlcN treatment) at both the (a) protein and (b) mRNA level after GlcN treatment.