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. 2014 Oct 30;13(12):3698–3708. doi: 10.1074/mcp.M114.043489

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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Fig. 3. — Transmission characteristics mass selection filter. A, isolation window efficiency comparison between the Q Exactive and Q Exactive Plus using the complete set of calmix ions. B, isolation transmission efficiency comparison between the Q Exactive and the Q Exactive HF using the complete set of calmix ions. C, total unique peptides from a complex HeLa total cell lysate, sequenced with a range of isolation windows. The secondary y-axis denotes the contribution of the Andromeda option “second peptide” as a percentage (SecPep). D, isolation purity from a complex HeLa total cell lysate, sequenced with a range of isolation windows.