Fig. 3.
Performance evaluation of the HD-DDA method at varying LC gradient times. A, Triplicate analysis of 1.000 ng of HeLa digest using gradient lengths from 15′ to 360′. B, Quantitative reproducibility of two 60′ gradient replicates based on peptide precursor intensity. C, Characteristics of the number of eluting (peptide) features (dashed line) as well as the rate of identification of eluting features and acquired tandem mass spectra at different LC gradient times. D, Qualitative reproducibility of two 60′ gradient replicates based on the number of identified proteins and peptides.