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Evaluation of the suitability of HD-DDA for PTM analysis and MS/MS based quantification. A, Analysis of IMAC enriched phosphopeptides in two technical replicates using 5 h total analysis time. B, TMT-quantification of the interaction of Trichostatin A with cellular proteins employing a competition binding assay using immobilized SAHA and increasing doses of Trichostatin A.
