Figure 5.
Influence of SPECTRA™ on “extracellular” superoxide (O2−) formation in blood cells collected from human volunteers. Superoxide formation was analyzed in human blood using EPR spectrometer nOxyscan, spin probe CMH (200 μmol/L) after addition of SOD (50 U/mL) in the blood samples taken prior to and at 60, 120, 180 min after consumption of standard breakfast (bread roll with glass of water). Blue columns (control): prior to and 60, 120, 180 min after consumption of standard breakfast (bread roll with glass of water); Red columns (placebo): after consumption of standard breakfast and placebo capsule; and Green columns (SPECTRA™): after consumption of standard breakfast and SPECTRA™ capsule. The values of superoxide generation were calculated as delta value between “total” and “SOD” sample. Data are mean ± SEM (n = 22), *P < 0.05 versus value “before.” CMH, 1-hydroxy-3-methoxycarbonyl-2.2.5.5-tetramethylpyrrolidine; EPR, electron paramagnetic resonance.