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. 2014 Nov 20;78(6):1441–1452. doi: 10.1111/bcp.12471

Figure 1.

Figure 1

GPER agonist G1 (1 μm)-mediated ERK activation and stimulation of apoptosis in vascular smooth muscle cells transduced with either GPER wild type or P16L adenoviral construct. (A) Effect on G1-mediated ERK activation in vascular smooth muscle cells transduced with increasing viral doses of either GPER or P16L adenovirus. (B) Effect on G1-mediated apoptosis in vascular smooth muscle cells transduced by increasing viral doses of either wild type GPER or P16L GPER adenovirus. Data represent percentage of control vs. (flag-tagged) GPER expression as assessed by immunoblots using the anti-flag antibody (M2) normalized to a common GPER control (a pool of GPER-transduced vascular smooth cell lysates). Each point represents the extent of G1-mediated effect for a specific level of GPER protein expression of either WT or GPER P16L. * P < 0.05 based on a comparison of fits (Prism 4.0, GraphPad Software, San Diego California). - - -, WT GPER; —, P16L GPER