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. 2014 Dec 5;4:7330. doi: 10.1038/srep07330

Figure 5. INPP5F inhibits STAT3 phosphorylation and nuclear translocation.

Figure 5

(A) Immunoblot analysis shows that INPP5F overexpression in glioblastoma cells (GSC923, GSC1228, and U87) inhibits phosphorylation of STAT3 (Y705) and INPP5F knockdown (GSC211 and GSC827) enhances its phosphorylation. (B) Immunoblot analysis of nuclear [N] and cytosol [C] fractions reveals that overexpression of INPP5F inhibits STAT3 nuclear translocation while INPP5F knockdown enhances the nuclear translocation of STAT3. (C) Control and INPP5F overexpressing GSC923 cells were treated with IL-6 (50 ng/ml) for 5 and 10 min, and immunoblot was performed with anti-p-STAT3 (Y705). Immunoblot analysis shows that INPP5F inhibits IL-6 induced phosphorylation of STAT3. (D) Confocal microscopy analysis verified that overexpression of INPP5F decreases STAT3 phosphorylation and nuclear translocation compared to control.