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. 2014 Aug 6;14:198. doi: 10.1186/s12870-014-0198-2

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© Chen et al. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Observation and statistics of starch granules diameter during development of seeds. A, Bright-field images of grain cross-sections stained with Fast Green and iodine allowing for the visualization of both intracellular proteins (green) and starch (blue-purple). The yellow arrows show A-granule starch, and the red arrows point to B-granule starch. B, Diameter of starch granules during development of seeds: comparison between A-granule starch granules of Chinese Spring (CS; common wheat) and those of Aegilops peregrina. DPA: days post-anthesis. C, Diameter of starch granules during development of seeds: comparison between B-granule starch granules of CS and of Brachypodium distachyon Bd21.