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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Nat Commun. 2014 Dec 4;5:5641. doi: 10.1038/ncomms6641

Figure 3. IL-2 and IFNγ mRNA detection in antigen-specific CD4 T cells.

Figure 3

(a) IL-2 and IFN-γ mRNA detection in CD4 T cells from PBMCs of an HIV-infected individual stimulated with a HIV-Gag peptide pool, CMV lysate or PMA/Ionomycin for 12 hours (one representative experiment out of five). (b) IFN-γ mRNA detection in combination with CD69 antibody staining from PBMCs of an HIV-infected subject left incubated with medium alone or stimulated with an HIV-Gag peptide pool, SEB or PMA/Ionomycin for 12 hours. (c) Combined detection of IFN-γ protein, using a 45 minute capture assay, and IFN-γmRNA in CD4 T cells from PBMCs of an HIV-infected person incubated with medium alone or stimulated with an HIV-Gag peptide pool, SEB or PMA/Ionomycin for 12 hours (one representative experiment out of five). (d) Collective data of IFN-γ mRNA detection after HIV-Gag peptide pool, CMV lysate or PMA/Ionomycin stimulation. Statistical analysis was performed using a Wilcoxon matched-pairs test.