Visualization of DNA damage via Rad52 and RPA foci. (A) Representative images of Rad52-YFP and RPA-CFP foci in exponentially growing cultures in YES media for designated genotypes. Number of nuclei analyzed for wild type, rad13∆, rad16-249, rhp14∆, and rhp14∆ rad16-249 is 1816, 689, 1131, 854, and 843, respectively. See Table S7 for standard error and confidence interval. (B) Distribution of nuclei containing 1, 2, or 3 + Rad52-YFP and/or RPA-CFP foci.