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. 2014 Sep 16;198(4):1483–1493. doi: 10.1534/genetics.114.167775

Figure 4.

Figure 4

Mutational analyses provide genetic evidence for a folded conformation of the Ndc80 complex in vivo. A fivefold dilution series of cells (Table S1) was plated and grown at the indicated temperatures. Two suppressor mutations (encoding N564I in Ndc80 and L344S in Nuf2) can individually rescue growth of ndc80-121 cells at the restrictive temperature (rows 6 and 12). Mutant alleles encode the following amino acid changes: ndc80-121 (Y465C I469Q), ndc80-125 (Y465C I469Q N564I), ndc80-126 (N564I), and nuf2-101 (L344S). In a heterozygous diploid, ndc80-121/ndc80-121 is temperature sensitive (row 4). The N564I intragenic suppressor mutation in Ndc80 can rescue the ndc80-121 phenotype in cis, but not in trans (compare rows 7 and 11). The ndc80-126 allele confers a slow-growth phenotype, which is rescued by the mutations in ndc80-121 (compare rows 6 and 10).