Protein kinases that were associated with both P-bodies and stress granules in stationary-phase cells. The colocalization of the four GFP-tagged protein kinases, Cdc28, Fus3, Cbk1, and Pbs2, with a reporter for either P-bodies (A, Edc3-mCh) or stress granules (B, Pbp1-mCh). (C) Quantitation of the colocalization data shown in A and B. (D) The frequency of foci formation was diminished by the presence of the hyperactive RAS2val19 allele. (E) Quantitation of the RAS2val19 effects on protein kinase foci formation.