Table 2. Oocytes prematurely enter M phase in lin-41 mutants.
| Genotype | Temperature | No. of proximal M-phase nucleia | % Fixed gonads with M-phase nucleia |
|
|---|---|---|---|---|
| Immediately after pachytene (%) | Proximally (%) | |||
| lin-41(n2914) | 20° | 14.2 ± 9.7 (n = 13) | 63 (n = 16) | 100 (n = 16) |
| lin-41(tn1505) | 20° | ND | 90 (n = 21) | 100 (n = 21) |
| lin-41(tn1487 ts)b | 25°c | 1.5 ± 1.4 (n = 20) | 25 (n = 20) | 85 (n = 20) |
| lin-41(tn1487 ts)b | 22°c | 1.2 ± 1.7 (n = 22) | 0 (n = 22) | 54 (n = 22) |
| lin-41(tn1487 ts)b | 15° | 0.2 ± 0.4 (n = 14) | 0 (n = 14) | 21 (n = 14) |
| lin-41(tn1487 ts)b | 22°d | 0.6 ± 0.6 (n = 24) | 0 (n = 24) | 54 (n = 24) |
| lin-41(tn1487 ts); oma-1(zu405te33); oma-2(te51)b | 22°d | 0 ± 0 (n = 27) | 0 (n = 27) | 0 (n = 27) |
| lin-41(tn1487 ts); fog-2(oz40)e | 22°f | 0.5 ± 0.8 (n = 28) | 0 (n = 28) | 32 (n = 28) |
a
Nuclei that have undergone NEBD and are positive for phospho-histone H3 (Ser10).
b
lin-41(tn1487 ts) homozygous parent.
c
Animals were upshifted from 15° as embryos.
d
Animals were upshifted from 15° as L4 larvae.
e
lin-41(tn1487 ts) heterozygous parent.
f
Animals were upshifted from 15° as L2–L4 larvae.