Figure 4.

Galactose sensitivity of gale-1(pv18). (A) The percentage (%) of animals reaching adulthood after 4 days of incubation at different concentrations of D-galactose. Dramatically fewer gale-1(pv18) animals reached adulthood at the highest concentration of D-galactose, whereas wild-type or gale-1(pv18) transgenic with gale-1(+) animals were hardly affected. The nonmetabolizable sugar L-glucose does not affect development of gale-1(pv18) at any concentration assayed. There are no significant differences between wild type at 0 and any galactose concentration. There are significant differences between gale-1(pv18) at 0 and 1–3 mg/ml of galactose. P < 0.1. (B) The percentage (%) of animals arrested in L1 at different concentrations of galactose, glucose, or mannose. Treatment with 0.5 mg/ml of any of the sugars generates a percentage of arrested animals. This percentage does not increase in higher concentrations of glucose or mannose (P > 0.1). At higher concentrations of galactose, the percentage of arrested animals increases (P < 0.05 for 0.5 mg/ml vs. any other concentration). (C) A representative L1-arrested animal in the presence of galactose. Bar, 15 μm. Data are from two different experiments with n ≥ 200 in each.