Figure 5.

Developmental defect phenotypes of gale-1(pv18). (A) Wild-type gonad, which exhibits a U-shape. (B) gale-1(pv18) gonad. In this mutant, the gonads exhibit an irregular shape and are not symmetrical as indicated by the arrow. (C) Percentage of abnormal gonads in wild-type (N2) and gale-1 mutant. n > 20. P < 0.05. Animals were grown at 20°. (D) Wild-type vulva at the L4 stage. (E) Vulva of an L4 gale-1(pv18) animal. Note the reduced size (see also Figure S1). Animals were grown at 20°. (F) Wild-type embryos incubated at 25°. (G) gale-1(pv18) embryos incubated at 25°. Note the bubbles of embryonic material and vacuoles in the mutant indicated by arrowheads (see also File S1). Bars, 40 μm (A and B); 2.5 μm (D and E); and 20 μm (F and G).