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. 2014 Oct 8;198(4):1559–1569. doi: 10.1534/genetics.114.170084

Figure 6.

Figure 6

MIG-17 location is altered in gale-1 and sqv-7 mutant backgrounds. (Top) GFP fluorescence. (Bottom) Nomarski. White arrows indicate the location of the basement membrane. (A) MIG-17::GFP is located in the basement membrane of the gonad in a wild-type background; 32 ± 10.6% of increase of fluorescence level in the basement membrane relative to the central region of the gonad. n = 8. (B) In a gale-1(pv18) mutant background, the location of MIG-17::GFP in the basement membrane is reduced relative to wild-type background; 10.2 ± 3.9% increase of fluorescence level in the basement membrane relative to the central region of the gonad. n = 8. The differences with the wild-type background are significant; P < 0.001.Two tailed t-tests were used for comparison. (C) Similarly, in an sqv-7(n2839) background, MIG-17::GFP is also mislocalized. (D) mig-23(k180), in which MIG-17::GFP has been reported to be mislocalized, was used as control. Bar, 20 μm.