Figure 8.

Tip60 HAT loss in the CNS causes misregulation of neuronal genes. (A) Real-time PCR was performed on cDNA isolated from adult fly heads expressing dTip60 ^E431Q under the elav C155 pan-neuronal GAL4 driver. Histogram represents relative fold change in expression level of neuronal target genes. Real-time PCRs were performed in triplicate, and the fold change was calculated using the ΔΔCT method. Statistical significance was calculated using an unpaired Student’s t-test: *P < 0.05 and **P < 0.01. (B) List of selected learning- and memory-related genes and their functions that are identified in ChIP-seq analysis and validated in adult head tissue using quantitative RT-PCR.