Figure 1.

(A–D) Mean (±SE) reproductive output of miRNA mutant females and their controls, relative to the estimate for reproductive output of virgin females of the same genotype. Reproductive output was scored as offspring counts either 24 or 48 hr after a single mating to SP-lacking (SP⁰, bars with dark shading) or SP-transferring (SP⁺, bars with light shading) males. Females were either hypomorphic for mir-279 or mir-317 in two different genetic backgrounds (A) w^[Dah] or (B) w^[CS]. (C) Results for knockout mir-278 in the w^[Dah] genetic background or (D) mir-184 in the w¹¹¹⁸ background. (E–H) Effect sizes $\left(\text{mean}\hspace{0.5em}S{P}^{+}\hspace{0.5em}-\hspace{0.5em}\text{mean}\hspace{0.5em}S{P}^{\mathit{0}}\right)/\text{S}{\text{D}}_{\text{pooled}}$ and 95% CI for reproductive output scored for the same female genotypes as in A–D 24 (diamonds with dark shading) or 48 hr (squares with light shading) after a single mating to either a SP⁺ or a SP⁰ male.