Figure 3.

Pbx1 EMSA. Biotin-labeled double-stranded oligonucleotide probes were incubated with nuclear proteins extracted from control or dehydrated SON. After electrophoresis, complexes were transferred to a membrane for ECL detection. A, Lane 1, probe only; lane 2, probe with control SON nuclear extract; lane 3, probe with dehydrated nuclear extract; lane 4, probe, control SON nuclear extract, and an excess of unlabeled oligonucleotide probe; lane 5, probe, dehydrated SON nuclear extract, and an excess of unlabeled oligonucleotide probe. The arrow indicates the position of a specific complex. B, Further independent EMSA experiments (control [C], 1–2; and dehydrated [D], 1–2) confirm an increase in Pbx1 complexes after dehydration.