Figure 7.

Secreted A1AT is decorated with paucimannosidic N-glycans. LC-ESI-MS of trypsin-digested A1AT collected from the IF. ^StrepA1AT was transiently expressed in N. benthamiana wild-type (WT; A) and glycosylation mutant (ΔXT/FT; B) plants. The N-glycosylation profile of glycopeptide2 (K/⁷⁰ADTHDEILEGLNFNLTEIPEAQIHEGFQELLR¹⁰¹) is shown. Peaks were labeled using the ProGlycAn system (www.proglycan.com). Adjacent illustrations display the respective N-glycans using standard symbols. m/z, Mass-to-charge ratio. [See online article for color version of this figure.]