Figure 8.
Arabidopsis HEXO3 generates paucimannosidic structures on secreted A1AT. LC-ESI-MS analysis of trypsin-digested A1ATFull expressed in Arabidopsis HEXO knockout lines (Liebminger et al., 2011). Glycan analysis was performed on the 40-kD A1AT collected from IF from hexo1 knockout (A), hexo3 knockout (B), and hexo1/hexo3 double knockout (C). Peaks were labeled using the ProGlycAn system (www.proglycan.com). Adjacent illustrations display the respective N-glycans using standard symbols. m/z, Mass-to-charge ratio. [See online article for color version of this figure.]