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. 2014 Oct 7;166(4):2077–2090. doi: 10.1104/pp.114.246371

Figure 5.

Figure 5.

PP2AB′1 acts downstream of ABA biosynthesis and perception during AM colonization. A, Quantification of AM colonization in the pp2aB′1-1 mutant transformed with abi1-1 or empty vector (EV) at 5 weeks after inoculation with R. irregularis. Expression of abi1-1 impaired AM colonization in the wild type (WT) similarly to pp2aB′1-1 expressing abi1-1. Values are means ± se (n = 10) from one biological replicate (for biological replicates 2 and 3, see Supplemental Fig. S17). The asterisk indicates significance in comparison with the wild type (Student’s t test; P ≤ 0.001). B, External application of ABA does not restore AM colonization in the pp2aB′1 mutant. AM colonization is shown in pp2aB′1-1 and sta-1 mutants watered with 0, 5, or 50 µm ABA for 4 weeks; the colonization is observed 4 weeks after R. irregularis inoculation. Asterisks indicate significant differences compared with the wild type watered with 0 µm ABA or with the wild type with the same ABA regime (Student’s t test; P < 0.001). Values are means ± se (n = 12). C, Bright-field images of wild-type as well as pp2aB′1-1 and sta-1 roots inoculated with R. irregularis for 4 weeks and watered with 0 µm ABA. The fungal structures are ink stained (blue coloration). Bars = 70 µm.