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. 2014 Oct 27;166(4):2115–2132. doi: 10.1104/pp.114.250647

Figure 3.

Figure 3.

Cytokinin responses. A, Three-week-old im and Col-0 plants were sprayed with 25 μm BAP every 3 d for a period of 2 weeks; shown are plants after treatment for 2 weeks. B, Time taken to achieve the floral transition in the plants in A. Values represent averages ± se of five independent replicates (***P < 0.001 relative to control plants). C, Total cell RNAs were isolated from im and Col-0 leaves that emerged after the 2-week BAP treatment, and real-time qRT-PCR analyses were performed to examine the expression of cytokinin response genes (ARR4–ARR7) and marker genes for the floral transition. Values are normalized to ACTIN and relative to control im and Col-0 and represent averages ± se of three technical and two biological replicates (***P < 0.001, **P < 0.01, *P < 0.05). D, Real-time qRT-PCR for cytokinin response genes (ARR4–ARR7) and cytokinin biosynthesis genes (IPT1 and IPT5) in Col-0, im, imgi2, and gi2 across all three developmental stages. The values are normalized to ACTIN and relative to Col-0 stage I plants for each gene and represent averages ± se of three technical and two biological replicates (***P < 0.001 and **P < 0.01 relative to Col-0 stage I plants). E, Representative im and detopped im plants approximately 7 weeks after germination; im was detopped at approximately 6 weeks.