Figure 7.

Photosynthetic protein accumulation. Total leaf proteins were isolated from the top two fully expanded rosette leaves of Col-0, im, imgi2, and gi2 at stages I to III. The proteins were loaded on an equal fresh weight basis for electrophoresis via 12% SDS-PAGE. Immunoblot analysis used polyclonal antibodies to the large subunit of Rubisco (LS), the light-harvesting complex protein (Lhcb2), the α-subunit of ATP synthase (ATPα), PSII subunit O (PsbO), PSI subunit F (PsaF), PSII reaction center protein (D1), and the Rieske Fe-S center of the cytochrome b₆f complex (Rieske Fe-S).