Table II. Examples of putative priming or potentiation of systemic Xtc-induced gene expression changes by primary/local Psj and Xtc infections.
Plants were treated in (local) leaf 1 with 10 mm MgCl2 (mock), Psj, or Xtc. Five days later, all treated plants were inoculated in (systemic) leaves 2 and 3 with Xtc, and gene expression was analyzed in these leaves at 24 h after Xtc inoculation using 44K barley microarrays (Agilent). Putatively primed regulation of gene expression is summarized for three transcripts with elevated accumulation after the systemic Xtc challenge infection of locally preinfected plants compared with control-treated plants (in boldface) and three transcripts with reduced accumulation after the systemic Xtc challenge infection of locally preinfected plants compared with control-treated plants (in lightface).
| Agilent Probe Identifier | GenBank Accession No. | Description of Barley Sequence (Genespring GX11; Agilent) | Average Normalized Expression at 24 h after Xtc Infection Systemic to the Primary/Local Treatmentsa |
Putatively Primed Regulation of Systemic Gene Expression in Response to Xtc by the Primary/Local Psj and Xtc Treatments Compared with the Primary/Local Mock Treatment |
|||||
|---|---|---|---|---|---|---|---|---|---|
| Mock (IM) | Psj (IP) | Xtc (IX) | IPIMb | Pa | IXIMc | Pa | |||
| A_13_P062881 | AJ462829 | Subspecies vulgare, mRNA | 63.03 | 180.74 | 175.90 | +2.87 | 0.0042 | +2.79 | 6.71E-05 |
| A_13_P102730 | BF262337 | Seedling green leaf EST, mRNA | 20.17 | 57.11 | 51.63 | +2.83 | 0.0082 | +2.56 | 0.0009 |
| A_13_P044391 | AJ485291 | mRNA | 141.01 | 380.32 | 291.35 | +2.70 | 0.001 | +2.07 | 0.0059 |
| A_13_P061976 | AJ467036 | mRNA | 33.23 | 13.97 | 18.79 | −2.38 | 5.48E-05 | −1.77 | 0.0004 |
| A_13_P030016 | BF256646 | Seedling root EST, mRNA | 146.20 | 55.30 | 86.97 | −2.64 | 0.0003 | −1.68 | 0.0093 |
| A_13_P018166 | BM816774 | Subspecies vulgare, mRNA, similar to hydroxymethylglutaryl-CoA reductase (NADPH), 4-α-glucanotransferase, hydroxymethylglutaryl-CoA reductase | 480.82 | 223.39 | 291.0 | −2.15 | 0.0007 | −1.65 | 0.0016 |
Data extracted from Supplemental Table S2. 2IPIM, Induced gene expression in Psj-pretreated compared with mock-pretreated plants. cIXIM, Induced gene expression in Xtc-pretreated compared with mock-pretreated plants.