Figure 6.
NMR lignin characterization. A, Two-dimensional HSQC NMR spectra of the lignin aromatic region of senesced stem material from the wild type, atr2-1, and atr2-2. The volume integrals of the S2/6, G2, and H2/6 contour peaks, when scaled (by 2 for G), represents the quantity of each monomer. B, Overview of the relative amounts of the different monolignol-derived units and the S/G ratio as determined from the HSQC aromatic region. C, Overview of the relative amount (as determined by uncorrected volume integrals) of interunit linkage types (β-O-4, β-5, and β-β) as determined from the HSQC side chain region (for the spectra of the side chain regions, see Supplemental Fig. S4). **0.01 > P > 0.001, ***P < 0.001; n = 3.