Table I. Metabolites with a reduced abundance in atr2 mutants.
An overview of the identified compounds of which the abundance was lower in the atr2-1 and atr2-2 mutants is given. For each compound, average peak areas of wild-type and atr2 mutant samples are given. For the structural elucidation of the compounds, see Supplemental Table S2 and Supplemental Figure S2.
No. | Retention Time | m/z | Name | Wild Type | atr2-1 | Change in atr2-1:Wild-Type Ratio | atr2-2 | Change in atr2-2:Wild-Type Ratio | |||
---|---|---|---|---|---|---|---|---|---|---|---|
Mean | se | Mean | se | Mean | se | ||||||
min | |||||||||||
Glucosinolates | |||||||||||
1 | 1.28 | 422.025 | 3-Methylsulfinylpropyl glucosinolate | 176,502 | 7,833 | 48,551 | 4,389 | 0.28 | 46,856 | 2,768 | 0.27 |
2 | 3.82 | 388.077 | 2-Hydroxy-3-butenylglucosinolate | 2,701 | 293 | 526 | 129 | 0.19 | 386 | 51 | 0.14 |
3 | 3.61 | 895.117 | Indol-3-ylmethylglucosinolate (dimer) | 10,432 | 1,004 | 3,219 | 456 | 0.31 | 2,997 | 273 | 0.29 |
4 | 2.10 | 847.149 | 4-Hydroxyindol-3-ylmethylglucosinolate (dimer)a | 2,194 | 129 | 926 | 173 | 0.42 | 1,064 | 159 | 0.49 |
Oligolignols | |||||||||||
5 | 8.84 | 375.145 | G(8-O-4)G | 274 | 58 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
6 | 9.14 | 375.146 | G(8-O-4)G | 222 | 57 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
7 | 12.97 | 339.125 | G(8-5)Ga | 2,063 | 288 | 33 | 17 | 0.02 | 33 | 12 | 0.02 |
8 | 15.45 | 337.109 | G(8-5)G'a | 877 | 79 | 13 | 9 | 0.02 | 81 | 27 | 0.09 |
9 | 12.80 | 369.136 | S(8-5)Ga | 208 | 41 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
10 | 13.12 | 553.208 | G(8-O-4)G(8-5)G | 356 | 71 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
11 | 13.42 | 553.209 | G(8-O-4)G(8-5)G | 574 | 65 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
12 | 14.71 | 583.218 | G(8-O-4)S(8-5)G | 7,656 | 817 | 78 | 44 | 0.01 | 51 | 25 | 0.01 |
13 | 15.52 | 583.219 | G(8-O-4)S(8-5)G | 2,158 | 301 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
14 | 16.25 | 581.203 | G(8-O-4)S(8-5)G' | 829 | 100 | 19 | 19 | 0.02 | 5 | 5 | 0.01 |
15 | 12.77 | 555.224 | G(8-O-4)G(red8-5)G | 456 | 80 | 0 | 0 | 0.00 | 5 | 5 | 0.01 |
16 | 16.66 | 613.232 | G(8-O-4)S(8-8)S | 37 | 15 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
17 | 16.96 | 583.219 | G(8-O-4)S/G(8-8)G/S | 1,260 | 233 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
18 | 18.00 | 809.306 | G(8-O-4)G(8-O-4)S(8-8)S | 272 | 63 | 0 | 0 | 0.00 | 0 | 0 | 0.00 |
Hexosylated oligolignols | |||||||||||
19 | 6.06 | 583.203 | G 4-O-hexoside(8-O-4)Gb | 523 | 80 | 24 | 18 | 0.05 | 46 | 30 | 0.09 |
20 | 7.61 | 613.212 | G 4-O-hexoside(8-O-4)Sb | 778 | 71 | 46 | 19 | 0.06 | 48 | 21 | 0.06 |
21 | 8.20 | 521.203 | G(red8-8)G hexoside | 412 | 60 | 42 | 17 | 0.10 | 53 | 28 | 0.13 |
22 | 9.04 | 521.202 | G(red8-8)G hexoside | 882 | 54 | 365 | 73 | 0.41 | 407 | 77 | 0.46 |
23 | 9.00 | 565.193 | G 4-O-hexoside(8-5)Gb | 3,748 | 298 | 576 | 91 | 0.15 | 1,053 | 136 | 0.28 |
24 | 9.14 | 567.208 | G 4-O-hexoside(red8-5)Gb | 2,975 | 321 | 182 | 81 | 0.06 | 286 | 91 | 0.10 |
25 | 10.68 | 521.203 | G(red8-5)G hexoside | 525 | 65 | 159 | 36 | 0.30 | 149 | 23 | 0.28 |
G(8-O-4/8-5)phenylpropenoic acid/ester | |||||||||||
26 | 5.98 | 551.177 | G(8-O-4)feruloyl hexose | 2,527 | 118 | 744 | 147 | 0.29 | 736 | 105 | 0.29 |
27 | 11.68 | 775.246 | G(8-O-4)feruloyl hexose + 224 D | 7,413 | 878 | 2,154 | 319 | 0.29 | 3,243 | 383 | 0.44 |
28 | 11.89 | 775.246 | G(8-O-4)feruloyl hexose + 224 D | 4,438 | 405 | 1,318 | 171 | 0.30 | 2,025 | 235 | 0.46 |
29 | 9.54 | 533.167 | G(8-5)feruloyl hexose | 492 | 39 | 0 | 0 | 0.00 | 16 | 10 | 0.03 |
30 | 10.08 | 533.166 | G(8-5)feruloyl hexose | 562 | 44 | 229 | 24 | 0.41 | 116 | 20 | 0.21 |
Compounds detected as fragments of the parent ion. bCompounds detected as formic acid adducts.