Table II. N-terminal sequences of the acidic and basic membrane-bound AtFNR1 and AtFNR2 proteins determined by Edman degradation.

The membrane proteins of Arabidopsis plants were separated by 2D gel electrophoresis and electroblotted, and the membrane was stained with Coomassie Brilliant Blue. The FNR spots were excised, and N-terminal sequencing was performed using the Procise 494A sequencer. The previously identified N-terminal sequences are also indicated. a denotes acidic form, and b denotes basic form. –, Sequence not detected.

Protein	N-Terminal Sequences	Reference
FNR1	VTTDTT	Hanke et al. (2005)
FNR1a	–	This study
FNR1b	QVTTDT	This study
	VTTDTT	This study
FNR2	ITTETD	Hanke et al. (2005)
FNR2a	–	This study
FNR2b	QITTETD	This study