Skip to main content
. 2014 Oct 24;166(4):2195–2204. doi: 10.1104/pp.114.251645

Figure 4.

Figure 4.

Down-regulation or overexpression of NUP1 in cultured tobacco BY-2 cells affects the expression of genes involved in the nicotine biosynthetic pathway and the accumulation of alkaloids. A to C, Quantitative RT-PCR analysis of NUP1 (A) and PMT, MATE1/MATE2, ERF189, MYC2, and PI-II (C) expression, and the amount of alkaloid in a culture flask (B) of wild-type, vector control (V1 and V2), NUP1 RNAi (R1 and R2), and NUP1 overexpressing (O1 and O2) BY-2 lines. Tobacco cells were treated with 100 μm MeJA for 24 h for RNA extraction and for 72 h for alkaloid analysis. The data are the mean values (±sd) of three biological replicates. Significant differences between the wild type and test samples were determined by Dunnett’s test and are indicated by asterisks (single asterisk for P < 0.05 and double asterisks for P < 0.01).