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. 2014 Oct 24;166(4):2195–2204. doi: 10.1104/pp.114.251645

Figure 5.

Figure 5.

Down-regulation of QPT does not affect the expression of genes involved in alkaloid biosynthesis and transport. Data from cultured tobacco BY-2 cells are shown in A to C, whereas data from tobacco hairy roots are shown in D to F. Quantitative RT-PCR analysis of QPT (A and D), PMT, BBL, MATE1/MATE2, and ERF189 (C and F) expression, and the accumulation of tobacco alkaloids (B and E) in wild-type, vector control (V1 and V2), and QPT RNAi (Q1, Q2, and Q3 in A–C, and Q1 and Q2 in D–F) transgenic lines. The cultured tobacco cells were treated with 100 μm MeJA for 24 h for RNA extraction and for 72 h for alkaloid analysis. The data are the mean values (±sd) of three biological replicates. Significant differences between the wild type and test samples were determined by Dunnett’s test and are indicated by asterisks (double asterisks for P < 0.01).