Figure 1.

RT-PCR screening of T-DNA insertion mutants. Total RNA was extracted from bam mutant Arabidopsis leaves and assayed for transcripts by RT-PCR as described in “Materials and Methods.” Wild-type (WT) RNA was used as a control. The double mutant bam5/3 was tested for the presence of both BAM3 and BAM5. Arrowheads indicate the PP2A internal control. Standard sizes are given in base pairs on the side. Expected amplicon sizes: BAM1, 484 bp; BAM2, 422 bp; BAM3, 807 bp; BAM5, 600 bp; BAM6, 716 bp; and PP2A, 232 bp.