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PCR genotyping of a segregating population of F2 plants from a Tsu-0 cross with emb3126-1. Primers were designed to amplify sequences adjacent to the ACC2 coding region in either Tsu-0 (top row) or “Nossen” (middle row). The bottom row shows the genomic region encoding 18S ribosomal RNA. SS, Plants homozygous for the “Nossen” allele of the suppressor; ST, heterozygotes; TT, plants homozygous for the Tsu-0 suppressor.
