Table II. Partial embryo rescue in F1 siliques from crosses between natural accessions and embryo-defective mutants.
| Mutant Allelea | Wild-Type Accession | Siliques Screened | Seeds Screened | Percentage Mutant Seeds | Percentage Mutant Seeds Exhibiting Embryo Rescue | Phenotype of Rescued Embryob | Average Size of Rescued Embryo |
|---|---|---|---|---|---|---|---|
| μm | |||||||
| emb3126-1 | Tsu-0 | 40 | 1,842 | 24.1 | 71.4 | Most large globularc | 84 |
| emb3137-1 | Tsu-0 | 40 | 1,939 | 24.3 | 75.4 | Large globular | 78 |
| emb3126-1 | Oy-0 | 11 | 474 | 24.5 | 72.4 | Small globular | 55 |
| emb3137-1 | Oy-0 | 20 | 965 | 26.5 | 75.6 | Small globular | 55 |
| emb3126-1 | Nie1-2 | 11 | 550 | 24.2 | Not determinedd | Tiny globular | 49 |
| emb3137-1 | Nie1-2 | 10 | 491 | 27.1 | Not determinedd | Tiny globular | 50 |
a
Embryo arrest in parental (“Nossen”) lines occurs at the preglobular stage.
b
Embryo rescue was more pronounced in crosses with a spectinomycin-tolerant accession (Tsu-0) than in crosses with spectinomycin-sensitive accessions (Oy-0 and Nie1-2).
c
Some embryos reached a later stage.
d
Rescued mutant seeds did not differ sufficiently in size from parental mutant seeds.