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. 2014 Aug 27;21(1):85. doi: 10.1186/s12929-014-0085-z

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© Incani et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Co-precipitation of endogenous SIN3A with HA-AIRE. COS-1 cells, containing endogenous SIN3A, were transfected with plasmid pEF5HA-AIRE and immunoprecipitated using SIN3A antibody. (line 3 and 4). In line 1 and 2 the empty pEF5HA vector and its total lysate are shown. Upper panel: W.B anti SIN3A. Lower panel: W.B anti AIRE HA. W.B.: Western blotting. I.P.: Immunoprecipitated. T.L.: Total Lysate.