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. 2014 Nov 30;14:287. doi: 10.1186/s12866-014-0287-2

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© Edgar et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The activity of FpvI and PvdS mutants in the absence of FpvR _1–89 . β-galactosidase assays were carried out with E. coli MC1061 (DE3) containing (A) pMP190::P_fpvA_lacZ or (B) pMP190::P_pvdE_lacZ, along with pETDuet expressing (A) mutant FpvI or (B) mutant PvdS. Dark grey bars: FpvI and PvdS mutants generated by error prone PCR. Patterned bars: FpvI and PvdS mutants engineered by site directed mutagenesis. Light grey bars: WT FpvI or PvdS. Data were obtained from three biological replicates and error bars are ±1 SD. Statistically significant difference to WT according to Student's T-test is indicated: *p <0.05, **p <0.01.