Table 1.
Bacterial strains and plasmids used in this study
| Strains | Genotype/phenotype | Reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild-type | [31] |
| PAO1 fpvR | PAO1 with fpvR (PA2388) deletion | [10] |
| PAO1 fpvR 1–89-TAP | PAO1 containing mini CTX2:: fpvR 1–89-TAP | This study |
| PAO1fpvR; pUCP23::fpvR 1–89-TAP | PAO1fpvR containing pUCP23::fpvR 1–89-TAP; GmR, CbR | This study |
| E. coli | ||
| S17-1 | hsd R hsdM + recA thi pro [integrated RP4-2-Tc::Mu, Km::Tn7]; Sm R Tp R | [32] |
| JM83 | F- ara Δ (lac-proAB) rpsL [Φ80, lacZΔM15] thi | [33] |
| MC1061 | ΔlacX74 Hsr− Hsm+ rpsL | [34] |
| MC1061 (DE3) | MC1061 lysogenized with λDE3 [lacI lacUV5-T7 gene 1] | This study |
| Plasmids | ||
| pUCP23 | lacI q lacZ(α-fragment) aacC1 ColE1 ori; RO1600 ori; Cb/AmpR, GmR | [35] |
| pUCP23::fpvR 1-89TAP | 519 bp PCR fragment containing the fpvR promoter and 5′end of fpvR, joined to a C-terminal TAP-tag and cloned into pUCP23 | This study |
| Mini-CTX2 | Self-proficient integration vector; TcR | [36] |
| Mini-CTX2::fpvR 1-89TAP | 519 bp PCR fragment containing the fpvR promoter and 5′end of fpvR, joined to a C-terminal TAP-tag and cloned into Mini-CTX2 | This study |
| pFLP2 | pRO1600 ori, sacB, flp recombinase; ApR/CbR | [37] |
| pMP190::PfpvA_lacZ | fpvA promoter cloned upstream of lacZ in pMP190; CmR | [6] |
| pMP190::PpvdE_lacZ | pvdE promoter cloned upstream of lacZ in pMP190; CmR | [30,38] |
| pETDuet | Dual expression vector; ApR | [39] |
| pETDuet::fpvR 1–89 | fpvR 1–89 cloned into MCS2 of pETDuet | This study |
| pETDuet::fpvI | fpvI cloned into MCS1 of pETDuet | This study |
| pETDuet::pvdS | pvdS cloned into MCS1 of pETDuet | This study |
| pETDuet::fpvI_fpvR 1–89 | fpvI cloned into MCS1 and fpvR 1–89 cloned into MCS2 of pETDuet | This study |
| PETDuet::pvdS_fpvR 1–89 | pvdS cloned into MCS1 and fpvR 1–89 cloned into MCS2 of pETDuet | This study |