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. 2014 Nov 21;15:386. doi: 10.1186/1471-2474-15-386

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© Huisman et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of cycle number on mRNA expression and protein level of collagen type I and III. The high cycle number (1000) caused elevated Collagen type I mRNA levels (p < 0.05). Pro-collagen type III protein levels (C) were upregulated in the 100 cycles regimen (p < 0.05, 24 h). The *indicates a significant difference of p < 0.05, the indicates ƚ significant difference of p < 0.01, the error bars indicate the standard error. All data points from each group were normalized to unstretched controls harvested at every time point.