Fig. 2.
High-galactose diet phenocopies dGALT-null movement and NMJ defects. (A) Differences in the time required for wandering L3 larvae to rollover from inverted to upright position: control (dGALTC2) and dGALT-null (dGALTΔAP2) flies were fed food that was either galactose-free or supplemented with 200 mM galactose. (B) Representative NMJs imaged with anti-horseradish peroxidase (HRP; green) and anti-Discs-large (DLG; red) in wandering L3 for the above four conditions. (C–E) Quantification of synaptic bouton number (C), branch number (D) and inter-bouton spacing distance (E) for all four conditions, normalized to appropriate controls. Sample size: ≥ten animals per genotype. Error bars show s.e.m. with significance indicated: *P<0.05, **P<0.01, ***P<0.001.