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. 2014 Oct 17;7(12):1365–1378. doi: 10.1242/dmm.017137

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — sgl overexpression prevents dGALT motor, NMJ and HRP glycosylation defects. (A) Representative NMJs imaged with anti-horseradish-peroxidase (HRP; green) and anti-Discs-large (DLG; red) for control (dGALT^C2), dGALT null with driver alone (dGALT^ΔAP2; UH1/+), sgl overexpression (UAS-sgl/UH1-Gal4) and dGALT null with sgl overexpression (dGALT; UAS-sgl/UH1-Gal4). (B) Normalized time required for the wandering L3 to rollover for all four genotypes. (C) Quantification of synaptic bouton number and (D) inter-bouton spacing distance. (E) Sample NMJs imaged with anti-Fasciclin-II (FASII; red) and anti-HRP (green). (F) Normalized quantification of HRP intensity. Sample size: ≥seven NMJs. Error bars show s.e.m. with significance indicated: *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, not significant (P>0.05, N.S.).