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. 2014 Dec 5;5:202. doi: 10.3389/fendo.2014.00202

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Copyright © 2014 Nagnan-Le Meillour, Vercoutter-Edouart, Hilliou, Le Danvic and Lévy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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One-dimensional native-PAGE of soluble proteins extracted from respiratory mucosa (RM) and vomeronasal mucosa (VNO) of pigs. The gel was stained with colloidal Coomassie blue. Wells were loaded for each tissue as follows: pubertal female (PB), pre-pubertal female (PPB), pubertal male (PB), and pre-pubertal male (PPB). Arrow: faster migrating band used as a standard for quantification and normalization of the samples. Relative optical density (in %) was calculated by using ImageJ software.