Table 1.
5′RACE |
3′RACE |
|||
---|---|---|---|---|
5′Primer | 3’ Primer | 5′Primer | 3′Primer | |
OBP | GeneRacer™ 5′primer | 3′OBP primer: TCACTTGGCAGGACAGTCATCTCT | 5′OBP primer: CACCCAGGAACCTCAACCTGAACA | GeneRacer™ 3′primer |
VEG | GeneRacer™ 5′primer | 3′VEG primer: CTAGTTCCCTCCTGGAGAGCAGGTTTCGCTTT | 5′VEG primer: CAGGAGTTCCCGGCCGTGGGGCA | GeneRacer™ 3′primer |
The sequence of the GeneRacer primers is available at www.invitrogen.com. Gene specific 5′ primers correspond to the first codons coding for the N-terminal of protein sequence (without signal peptide) and gene specific 3′ primers to the last codons of the C-terminal of the protein sequence.