Skip to main content
. 2014 Dec 5;5:202. doi: 10.3389/fendo.2014.00202

Table 1.

Primers used in the RACE-PCR experiments for amplification of putative OBP and VEG splice variants.

5′RACE
3′RACE
5′Primer 3’ Primer 5′Primer 3′Primer
OBP GeneRacer™ 5′primer 3′OBP primer: TCACTTGGCAGGACAGTCATCTCT 5′OBP primer: CACCCAGGAACCTCAACCTGAACA GeneRacer™ 3′primer
VEG GeneRacer™ 5′primer 3′VEG primer: CTAGTTCCCTCCTGGAGAGCAGGTTTCGCTTT 5′VEG primer: CAGGAGTTCCCGGCCGTGGGGCA GeneRacer™ 3′primer

The sequence of the GeneRacer primers is available at www.invitrogen.com. Gene specific 5′ primers correspond to the first codons coding for the N-terminal of protein sequence (without signal peptide) and gene specific 3′ primers to the last codons of the C-terminal of the protein sequence.