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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Nature. 2012 Nov 7;492(7427):104–107. doi: 10.1038/nature11589

Figure 1. Wnt signaling promotes ocellus formation.

Figure 1

a, Gastrula stage schematic indicates the lineage specific expression of enhancers in this study. b, Tailbud electroporated with Mitf>LacZ detected with an antibody (green), and hybridized with a Wnt7 probe (red). c-f, Larvae electroporated with βγ-crystallin>GFP marks the otolith and anterior palps. c, Co-electroporated with Mitf>LacZ (166/196 had an otolith and ocellus). d, Co-electroporated with Mitf>Wnt7 (172/205 had two ocelli). e, Co-electroporated with Mitf>stable β-catenin (189/205 had two ocelli). f, Coelectroporated with Mitf>dnTCF (100/205 had two otoliths). g,h, Larvae electroporated with Zicl>Ets:VP16 and βγ-crystallin>GFP. g, Co-electroporated with Mitf>LacZ (75/100 had extra otoliths). h, Co-electroporated with Mitf>Wnt7 (only 11/100 had extra otoliths). Scale bars, 50 μm.