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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Curr Biol. 2012 Nov 1;22(23):2247–2252. doi: 10.1016/j.cub.2012.09.051

Fig. 2. Developmental consequences of removing the Hb stripe enhancer.

Fig. 2

Hb-mutant embryos carrying either a mutant BAC transgene lacking the stripe enhancer (A, C, E, F) or wild-type BAC transgene (B, D, G, H). Both transgenes contain 44 kb of genomic DNA encompassing the Hb transcription unit and flanking sequences. cc14 embryos were stained with antibodies against Hb (red) and even-skipped (green). The wild-type BAC directs a normal Hb expression pattern, and even-skipped (eve) pattern (B). In contrast, the mutant BAC transgene lacking the stripe enhancer exhibits reduced Hb expression in anterior regions, and loss of the posterior stripe (A; compare with B). The eve pattern is also altered, with expanded patterns of stripes 3 and 7 (A). During germband elongation, the wild-type BAC transgene directs normal stripes of engrailed (en) expression (D), whereas the mutant BAC lacking the stripe enhancer exhibits irregular spacing between en stripes 4 and 5 (C). The wild-type BAC transgene also produces completely normal cuticles (G, H), whereas the mutant BAC results in the variable loss of ventral mesothoracic (T2) pattern elements (E, F) and fusion of A7/A8 (E). eve and en expression patterns were diagnostic of genotype, clearly discernible by eye, and this was confirmed by staining for the presence or absence of a labeled balancer.