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Table 1.

Characterization of ovaries and fruits sampled at anthesis and MG stage

Anthesis MG stage
Pruned experiment Competition experiment Pruned experiment
Basal ovaries Tip ovaries Basal fruits Tip fruits Basal fruits Tip fruits
Cell number (106) CF14-L 0·22 ± 0·02 0·16 ± 0·01 0·66 ± 0·04 0·63 ± 0·10 0·50 ± 0·01 0·48 ± 0·11
CF12-C 0·15 ± 0·02 0·14 ± 0·01 0·62 ± 0·04 0·61 ± 0·05 0·63 ± 0·22 0·57 ± 0·12
Pericarp thickness (mm) CF14-L 0·09 ± 0·02 0·105 ± 0·01 2·02 ± 0·16 1·90 ± 0·01 2·07 ± 0·29 2·28 ± 0·32
CF12-C 0·07 ± 0·00 1·94 ± 0·56 1·64 ± 0·15 2·01 ± 0·14 2·27 ± 0·25
No. of cell layers CF14-L 9·5 ± 1·5 9·0 ± 1·0 18·8 ± 1·5 17·8 ± 1·5 15·5 ± 1·0 16·7 ± 0·7
CF12-C 8·5 ± 0·5 16·8 ± 1·6 16·3 ± 0·3 17·3 ± 1·8 16·8 ± 1·2
Cell area CF14-L 99 ± 4 107 ± 25 0·019 ± 0·002 0·019 ± 0·001 0·021 ± 0·002 0·025 ± 0·007
Anthesis (µm2) CF12-C 98 ± 1 0·020 ± 0·006 0·014 ± 0·002 0·020 ± 0·001 0·025 ± 0·005
MG (mm2)

The number of pericarp cells (106) was measured after tissue break down. The pericarp thickness, number of cell layers and mean cell area were defined by in situ observations of an ROI delimited by the pericarp thickness and a length of 350 µm and 2·5 mm at anthesis and MG stage, respectively. Each measurement of cell number is the mean of six cell counting per fruit, and data are means of 12 ovaries for the anthesis stage, and four and three MG fruit replicates in competition and pruned experiments, respectively, ± 95 % confidence intervals. In situ observations are means of two or three replicates ± 95 % confidence intervals.