Table 1. Aberrant tissue mineralization in Enpp1+/+, Enpp1+/asj-2J and Enpp1asj-2J mice on normal and acceleration diet compared with Enpp1asj mice on acceleration diet.
Mouse | Number ofmice examined | Soft tissue mineralization (%) | ||||||||
Vibrissae | Liver | Kidney | Heart | Aorta | Eyes | Carotidartery | Lung | Spleen | ||
Normal diet: | ||||||||||
Enpp1+/+ | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Enpp1+/asj-2J | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Enpp1asj-2J | 12 | 100 | 8 | 100 | 100 | 67 | 33 | 83 | 92 | 67 |
Acceleration diet: | ||||||||||
Enpp1+/+ | 8 | 0 | 0 | 88 | 0 | 0 | 0 | 0 | 0 | 0 |
Enpp1+/asj-2J | 8 | 0 | 0 | 100 | 0 | 0 | 0 | 0 | 0 | 0 |
Enpp1asj-2J | 6 | 100 | 100** | 100 | 100 | 100 | 100* | 100 | 100 | 100 |
Acceleration diet: | ||||||||||
Enpp1asj | 9 | 100 | 56 | 100 | 67 | 56 | 44+ | 78 | N/A | N/A |
Mice were placed on either normal diet or acceleration diet at 4 weeks of age, and tissues were collected for histopathology at 3 months of age. The values represent the number of mice, as percent of all animals examined, affected by mineralization as examined by hematoxylin and eosin stain on one section. Statistical analyses were performed with Fisher’s Exact test. Comparison to Enpp1asj-2J mice on normal diet: *P<0.05; **P<0.01. Comparison to Enpp1asj-2J mice on acceleration diet: + P<0.05.