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. 2014 Dec 5;9(12):e112873. doi: 10.1371/journal.pone.0112873

Figure 1. HmBRI size-exclusion chromatography (SEC) elution profile and visual light absorbance spectra in different environments.

Figure 1

(A) SEC elution profile of HmBRI. Two distinct peaks are observed, that correspond supposedly to monomeric HmBRI (at 73.4 ml, peak ratio is ∼1.95) and oligomeric HmBRI (at 67.8 ml, peak ratio is ∼1.85). Five 2 ml fractions (1–5) were collected for subsequent crystallization. (B) Spectra of the light-adapted HmBRI in the hexagonal crystals, the first and the fifth SEC fractions.