Figure 5. Phylogenetic tree and sequence alignment of HsBR, ar-1, ar-2, dr-3 and HmBRI.

The unique HmBRI D-E loop elongation, the residues that result in novel inter-helical hydrogen bonds, and the P50_Hs/T48_Hm position are highlighted. The propeptide residues that are cleaved in the mature protein in vivo are shown in grey. The asterisk indicates the fully conserved residues, the colon and period indicate conservation between groups of strongly similar properties scoring> 0.5 and < =  0.5 correspondingly in the Gonnet PAM 250 matrix [49].