Figure 5. oxtl, tphd2 and crh gene expression in cnot8^m1061 embryos at 3 dpf.

(A, B) oxtl, (C, D) tphd2 and (E–H) crh WISH expression analysis in wild-type siblings and cnot8^m1061 mutants. (A–F) lateral views. (G, H) dorsal views. Boxes indicate areas of cell counts. Sibling WT embryos developed on average 34.6 crh neurons and cnot8^m1061 mutants developed on average 59.4 crh neurons. Significance was evaluated by Mann-Whitney test (p = 0.008). Embryos were genotyped by PCR. Scale bar 100 µm.