Figure 3. Inter-specific GISH of triatomine species using three genomic DNA (gDNA) probes: Triatoma delpontei (TD) (male or female) and Triatoma infestans (TI) (Andean group).

Bar  = 5 µm. (a–b) TD gDNA on Triatoma dimidiata chromosomes. Metaphase II (2n = 20A + X₁X₂Y). (a) FITC (b) Merged. All chromatin present dispersal hybridization signals but only the Y chromosome exhibits a very strong labeled. (c) TI (Andean group) gDNA on T. carrioni chromosomes. Metaphase I (2n = 20A + XY). Strong hybridization signals are restricted to the heterochromatic Y chromosome. (d) TD gDNA on T. protracta chromosomes. Metaphase II (2n = 20A + X₁X₂Y). Only the Y chromosome presents strong hybridization signal in spite of the autosomal C-bands in all autosomal pairs and in one X chromosome. (e) T. protracta. C-banding. Metaphase II. All autosomal pairs, the Y chromosome and one X chromosome present C-blocks in one or both chromosomal ends. (f) TD gDNA on Dipetalogaster maxima chromosomes. Spermatogonial mitotic prometaphase with 22 chromosomes (2n = 20A + XY). Only the Y chromosome presents intense hybridization signal. (g) TI (Andean group) gDNA on Eratyrus mucronatus chromosomes. Metaphase I (2n = 20A + X₁X₂Y). Only the Y chromosome presents hybridization signal. (h) TI (Andean group) gDNA on Panstrongylus geniculatus chromosomes. Anaphase II (2n = 20A + X₁X₂Y) showing a post-reductional sex chromosomes segregation. Only the Y chromosome has strong hybridization signals. (i) TI (Andean group) gDNA on Rhodnius prolixus chromosomes. Metaphase I (2n = 20A + XY). The hybridization signals are scattered throughout all chromosomes. No chromosomal region was observed with intense labeled signals, including the heterochromatic Y chromosome.